AKT 1/2/3 antibody (AA 313-317)

Catalog No. ABIN363068

This Rabbit Polyclonal antibody specifically detects AKT 1/2/3 in IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for AKT 1/2/3 antibody (AA 313-317) (ABIN363068)

Target: AKT 1/2/3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT 1/2/3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-AKT 1/2/3 Antibody

Binding Specificity: AA 313-317

Specificity: The antibody detects endogenous level of total AKT1/AKT2/AKT3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 313-317, 314-318, 310-314 (P-E-Y-L-A) derived from Human AKT1, AKT2, AKT3. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for AKT 1/2/3

Target: AKT 1/2/3

Alternative Name: AKT1/AKT2/AKT3

Background: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. /IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

Molecular Weight: 60 kDa

UniProt: Q9Y243