EPAS1 antibody (AA 535-631)

Catalog No. ABIN363209

The Mouse Monoclonal anti-EPAS1 antibody has been validated for WB, IHC, ELISA, IP, ICC, FACS, IF and ChIP. It is suitable to detect EPAS1 in samples from Human, Rat, Mouse and Hamster.

Quick Overview for EPAS1 antibody (AA 535-631) (ABIN363209)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Human, Rat, Mouse, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Clone: Ep190b

Product Details anti-EPAS1 Antibody

Binding Specificity: AA 535-631

Specificity: Reacts with HIF-2 alpha from human, mouse hamster and rat

Cross-Reactivity (Details): Does not cross-react with HIF-1 alpha.

Purification: Purified (protein G)

Immunogen: Human HIF-2 alpha amino acids 535-631

Isotype: IgG1

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ELISA1:100-1:2000, FC1:400, IHC1:150-1:300, WB1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, Sodium azide 0.1 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: Hypoxia-Inducible Factor 2-alpha

Background: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

Gene ID: 2034

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect