ARNT antibody

Catalog No. ABIN363326

The Rabbit Polyclonal anti-ARNT antibody has been validated for WB, IHC, IP and ChIP. It is suitable to detect ARNT in samples from Human, Mouse, Rat, Cow, Sheep and Ferret.

Quick Overview for ARNT antibody (ABIN363326)

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Ferret

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARNT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Product Details anti-ARNT Antibody

Specificity: Reacts with human HIF 1 beta /ARNT

Cross-Reactivity: Mouse (Murine), Cow (Bovine), Sheep (Ovine), Rat (Rattus)

Cross-Reactivity (Details): Cross reacts with proteins from bovine, sheep, mouse, rat and ferret. Not tested in other species.

Purification: Antiserum

Immunogen: Fusion protein to human HIF-1 beta

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ChIP1:10-1:500 ICC/IF (Negative), IHC1:150, IP1:10-1:500, WB1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: , Sodium azide 0.02 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Alternative Name: Hypoxia-Inducible Factor 1-beta

Background: Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.

Gene ID: 405

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect