APEX1 antibody

Catalog No. ABIN363379

This Rabbit Polyclonal antibody specifically detects APEX1 in WB, IHC, IF, ICC, ChIP, IP and Neut. It exhibits reactivity toward Human, Mouse, Rat and Rabbit.

Quick Overview for APEX1 antibody (ABIN363379)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Neutralization (Neut)

Product Details anti-APEX1 Antibody

Specificity: Reacts with human ~37 kDa APE-1 protein

Cross-Reactivity: Mouse (Murine), Rabbit, Rat (Rattus)

Cross-Reactivity (Details): Cross reacts with proteins from mouse, rat and rabbit.

Purification: Antigen affinity purified

Immunogen: Affinity purified human Apurinic/Apyrimidinic Endonuclease (APE/ref-1) fusion protein

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
- IHC: 1/1 000 - WB : 1/1 000 - B/N: 50-200 Molar excess - ChIP: 1/10-1/500 - ICC/IF: 1/50-1/200 - IP: 7 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Tris-glycine, NaCl 150 mM buffer, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: DNA-(Apurinic Or Apyrimidinic Site) Lyase (APE-1)

Background: Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents.

Gene ID: 328

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus