EPAS1 antibody (C-Term)

Catalog No. ABIN363383

The Rabbit Polyclonal anti-EPAS1 antibody has been validated for WB, IHC, ELISA, IP, ICC, FACS, IF, ChIP and GS. It is suitable to detect EPAS1 in samples from Human, Rat, Mouse and Fish.

Quick Overview for EPAS1 antibody (C-Term) (ABIN363383)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Human, Rat, Mouse, Fish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS)

Product Details anti-EPAS1 Antibody

Binding Specificity: C-Term

Specificity: Reacts with mouse, rat, fish and human HIF-2 alpha

Cross-Reactivity (Details): Does not cross-react with HIF-1 alpha.

Purification: Antigen affinity purified

Immunogen: Synthetic peptide derived from the C-term domain of mouse/human HIF-2 alpha protein.

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
WB1.0 μg/mL - 2.0 μg/mL ChIP1:10-1:500 ELISA 1:100-1:2000 ICC/IF1:100 IHC 1:100 IP5 μg/1 mg lysate

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: Hypoxia-Inducible Factor 2-alpha

Background: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung.

Gene ID: 2034

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect