EGLN1 antibody (AA 1-50)

Catalog No. ABIN363411

This anti-EGLN1 antibody is a Rabbit Polyclonal antibody detecting EGLN1 in WB, IHC, IP and FACS. Suitable for Human.

Quick Overview for EGLN1 antibody (AA 1-50) (ABIN363411)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-EGLN1 Antibody

Binding Specificity: AA 1-50

Specificity: Reacts with human HPH-2

Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Does not react in mouse. Not tested in other species

Purification: Antigen affinity purified

Immunogen: Synthetic peptide between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_071334.1 (GeneID 54583).

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
FC 1:10-1:1000, WB 1:500-1:2500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 Mg/M

Buffer: Tris-citrate/phosphate, pH 7-8, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: Hypoxia-Inducible Factor Prolyl Hydroxylase 2

Background: HIF-PH2, under normoxic conditions,catalizes the post-translational formation of 4-hydroxyproline on HIF-alpha leading to its proteasomal degradation. This antibody has been tested on human glioblastoma cell lysates.

Gene ID: 54583

Pathways: cAMP Metabolic Process, Warburg Effect