HIF1A antibody (AA 530-825)

Catalog No. ABIN363439

Product Details anti-HIF1A Antibody

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Binding Specificity: AA 530-825

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Specificity: Reacts with HIF-1 alpha from Human, Mouse, Rat, Goat, Primate and Rabbit

Purification: Antigen affinity purified

Immunogen: Fusion protein including residues 530-825 of the mouse HIF-1 alpha protein

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
WB(1:500 - 1:2000) ICC/IF(1:100) IHC (5 - 10 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: , Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: Hypoxia-Inducible Factor 1-alpha (HIF1A Products)

Background: Upon hypoxia, this proteins regulates over 40 genes by activating their transcription.

Gene ID: 15251

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect