HMGB1 antibody (AA 100-200)

Catalog No. ABIN363507

This anti-HMGB1 antibody is a Rabbit Polyclonal antibody detecting HMGB1 in WB, IHC, IF, FACS and ICC. Suitable for Human, Mouse, Rat, Cow, Dog and Sheep.

Quick Overview for HMGB1 antibody (AA 100-200) (ABIN363507)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-HMGB1 Antibody

Binding Specificity: AA 100-200

Specificity: Reacts with HMGB1 protein from Human, Mouse, Rat, Bovine, Canine, Sheep

Cross-Reactivity (Details): predicted to cross react with Equine, Rabbit, Porcine (100 % ).

Purification: Purified

Immunogen: Synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200)

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
WB(0.5 - 1.0 μg/mL) FC(1:100) ICC/IF(0.05 μg/mL) IHC (1:100-1:250)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.06 mg/mL

Buffer: Tris-glycine, NaCl 150 mM buffer, Sodium azide 0.1 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 6 months

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Background: HMGB1 is a DAMP involved in innate immune response. It is secreted by activated dendritic cells, macrophages and necrotic cells.

Gene ID: 3146

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome