LC3B antibody (N-Term)

Catalog No. ABIN363542

The Rabbit Polyclonal anti-LC3B antibody has been validated for WB, IHC, IF, FACS, ICC and IEM. It is suitable to detect LC3B in samples from Human, Mouse, Rat and Cow.

Quick Overview for LC3B antibody (N-Term) (ABIN363542)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)

Product Details anti-LC3B Antibody

Binding Specificity: N-Term

Specificity: Reacts with LC3B from human, mouse, rat and bovine and Zebrafish

Cross-Reactivity (Details): Other species have not been tested.

Purification: Antigen affinity purified

Immunogen: Synthetic peptide made to the N-terminal region of the human LC3, isoform B protein

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ICC/IF(1:2000) FC (1:200) IHC (1:400) WB (1:1000)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: Light Chain 3 beta (LC3B)

Background: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Gene ID: 81631

Pathways: Autophagy