S100A8 antibody (FITC)

Catalog No. ABIN364230

Product Details anti-S100A8 Antibody (FITC)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Anti-Human Calgranulin A monoclonal antibody reacts with the human Calgranulin A protein. Calgranulin A, also known as MRP-8 and S 100A8 is a member of the S 100 family of proteins containing 2 EF hand (alpha helix, turn, alpha helix structure) calcium bi.

Purification: Protein G purified

Immunogen: Purified granulocyte antigen of human origin.

Clone: CF-145

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo (0.5 μg/10 x10⁶), IF, IHC, IHC-P (1:50), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, EIA grade BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8 / MRP8 (S100A8 Products)

Background: Name/Gene ID: S100A8

Synonyms: S100A8, 60B8AG, CFAG, CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, CGLA, L1Ag, NIF, p8, Protein S100-A8, MRP8, Urinary stone protein band A, CP-10, MA387, MRP-8

Gene ID: 6279

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins