KDM4C antibody (AA 475-525)

Catalog No. ABIN364250

The Rabbit Polyclonal anti-KDM4C antibody has been validated for WB, IHC and IHC (p). It is suitable to detect KDM4C in samples from Human.

Quick Overview for KDM4C antibody (AA 475-525) (ABIN364250)

Target: KDM4C (Lysine (K)-Specific Demethylase 4C (KDM4C))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM4C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KDM4C Antibody

Binding Specificity: AA 475-525

Brand: IHC-plus™

Specificity: This antibody is specific to the JMJD2C protein. Species cross-reactivity: Human. Other species not yet tested.

Purification: Immunoaffinity purified

Immunogen: The epitope recognized maps to a region between residue 475 and 525 of human Jumonji Domain Containing 2C using the numbering given in entry NP_055876.2 (GeneID 23081).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: HeLa whole cell extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7.5, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for KDM4C

Target: KDM4C (Lysine (K)-Specific Demethylase 4C (KDM4C))

Alternative Name: KDM4C / JMJD2C

Background: Name/Gene ID: KDM4C

Synonyms: KDM4C, BA146B14.1, GASC-1 protein, GASC1, Jumonji domain containing 2C, JMJD2C, JHDM3C, KIAA0780, Lysine-specific demethylase 4C

Gene ID: 23081

NCBI Accession: NP_055876

UniProt: Q9H3R0

Pathways: Nuclear Hormone Receptor Binding, Warburg Effect