C4B antibody

Catalog No. ABIN364282

This Chicken Polyclonal antibody specifically detects C4B in WB, IHC, ELISA, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for C4B antibody (ABIN364282)

Target: C4B (C4b) (Complement Component C4b (C4b))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This C4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-C4B Antibody

Brand: IHC-plus™

Specificity: Anti-Human Complement Component C4b polyclonal antibody reacts with human C4b. C4 is a 210 KDa trimeric glycoprotein containing three polypeptide chains alpha, beta, and gamma with respective molecular weights of 93KDa, 75KDa and 33 KDa. C4 breaks down in.

Purification: Purified

Immunogen: Purified human complement C4b.

Isotype: IgY

Application Details

Application Notes: Approved: ELISA, Flo (0.1 μg/10 x10⁶), IHC, IHC-P (20 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

Target Details for C4B

Target: C4B (C4b) (Complement Component C4b (C4b))

Alternative Name: Complement C4B

Background: Name/Gene ID: C4B

Synonyms: C4B, C4B12, C4BD, C4B1, C4B3, Complement C4B, CH, Chido form of C4, CPAMD3, Complement C4-B, CO4, Basic complement C4, C4B2, C4B5, C4F, Complement C4B1a, Complement component 4B

Gene ID: 721

Pathways: Complement System