Claudin 7 antibody (C-Term)

Catalog No. ABIN364312

This Mouse Monoclonal antibody specifically detects Claudin 7 in WB, ELISA, IHC, IHC (p) and IP. It exhibits reactivity toward Human.

Quick Overview for Claudin 7 antibody (C-Term) (ABIN364312)

Target: Claudin 7 (CLDN7)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Claudin 7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-Claudin 7 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Specific for the claudin-7 protein and does not cross-react with Claudin-3 or -4. On Western blots, it identifies a band at ~22kD. Reactivity has been confirmed with T47D cells by Western blotting and with formalin-fixed, paraffin-embedded human breast cancer tissues by immunohistochemistry.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the C-terminal region of the human Claudin-7 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (10 μg/mL), IP, WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: Western Blot: T47D cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Claudin 7

Target: Claudin 7 (CLDN7)

Alternative Name: CLDN7 / Claudin 7

Background: Name/Gene ID: CLDN7
Family: Claudin

Synonyms: CLDN7, Claudin-7, Claudin-1, CLDN-7, Hs.84359, CEPTRL2, Claudin 7, CPETRL2

Gene ID: 1366

UniProt: O95471

Pathways: Hepatitis C