VAMP2 antibody (AA 1-89)

Catalog No. ABIN364330

This Mouse Monoclonal antibody specifically detects VAMP2 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for VAMP2 antibody (AA 1-89) (ABIN364330)

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VAMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3E5

Product Details anti-VAMP2 Antibody

Binding Specificity: AA 1-89

Brand: IHC-plus™

Purification: Protein G purified

Immunogen: Recombinant human Synaptobrevin 2 (1-89aa) purified from E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (1:2000 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.02 % sodium azide, 10 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for VAMP2

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Alternative Name: VAMP2 / VAMP-2

Background: Name/Gene ID: VAMP2
Family: Synaptobrevin

Synonyms: VAMP2, SYB2, Synaptobrevin 2, VAMP-2, Synaptobrevin-2

Gene ID: 6844

UniProt: P63027

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport