CD44 antibody (AA 21-145)

Catalog No. ABIN364348

This Mouse Monoclonal antibody specifically detects CD44 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD44 antibody (AA 21-145) (ABIN364348)

Target: CD44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5C10

Product Details anti-CD44 Antibody

Binding Specificity: AA 21-145

Purification: Protein G purified

Immunogen: Recombinant human CD44 (21-145aa) purified from E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: Name/Gene ID: CD44

Synonyms: CD44, CDW44, CD44 antigen, Cell surface glycoprotein CD44, CSPG8, ECMR-III, Epican, HUTCH-I, LHR, MDU2, MC56, MIC4, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, PGP-I, Pgp1, Phagocytic glycoprotein I, MDU3, CD44R, HCELL, Heparan sulfate proteoglycan

Gene ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling