Cofilin antibody (AA 1-166)

Catalog No. ABIN364365

This anti-Cofilin antibody is a Mouse Monoclonal antibody detecting Cofilin in WB, IHC, ELISA and IHC (p). Suitable for Human.

Quick Overview for Cofilin antibody (AA 1-166) (ABIN364365)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AT1C1

Product Details anti-Cofilin Antibody

Binding Specificity: AA 1-166

Purification: Protein G purified

Immunogen: Recombinant human Cofilin 1 (1-166aa) purified from E. coli. NCBI Accession No.: NP_005498.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: CFL1 / Cofilin

Background: Name/Gene ID: CFL1

Synonyms: CFL1, 18 kDa phosphoprotein, Cofilin, CFL, Cofilin 1 (non-muscle), Cofilin, non-muscle isoform, p18

Gene ID: 1072

NCBI Accession: NP_005498

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events