ACTH antibody (N-Term)

Catalog No. ABIN364371

The Mouse Monoclonal anti-ACTH antibody (Clone 57) (ABIN364371) specifically detects ACTH in IHC, ELISA and IHC (p). The antibody is reactive with Human and Rat samples.

Quick Overview for ACTH antibody (N-Term) (ABIN364371)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTH antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 57

Product Details anti-ACTH Antibody

Binding Specificity: N-Term

Specificity: Recognizes human Synacthen (1-24 ACTH). No crossreactivity to CLIP (17-39 ACTH). Species Crossreactivity: rat

Purification: Protein A purified

Immunogen: ACTH (KLH). Epitope: N-terminal.

Type of Immunogen: Protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Alternative Name: ACTH

Target Type: Hormone

Background: Name/Gene ID: ACTH

Synonyms: ACTH

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity