Leptin Receptor antibody (AA 829-841)

Catalog No. ABIN364407

This anti-Leptin Receptor antibody is a Goat Polyclonal antibody detecting Leptin Receptor in WB, ELISA, IHC and IHC (p). Suitable for Human.

Quick Overview for Leptin Receptor antibody (AA 829-841) (ABIN364407)

Target: Leptin Receptor (LEPR)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Leptin Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Leptin Receptor Antibody

Binding Specificity: AA 829-841

Brand: IHC-plus™

Specificity: Human LEPR / Leptin Receptor. This antibody is expected to recognise all three reported isoforms (NP_002294.2, NP_001003679.1, NP_001003680.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Marmoset, Panda, Dog, Horse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TQDDIEKHQSDAG from an internal region of human LEPR / Leptin Receptor (NP_002294.2, NP_001003679.1, NP_001003680.1). Percent identity by BLAST analysis: Human, Gorilla (100%), Marmoset, Panda, Dog, Horse (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (4 μg/mL), Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Leptin Receptor

Target: Leptin Receptor (LEPR)

Alternative Name: LEPR / Leptin Receptor

Background: Name/Gene ID: LEPR

Synonyms: LEPR, CD295 antigen, HuB219, LEP-R, OB receptor, LEPRD, OB-R, OBR, CD295, DB, Leptin receptor

Gene ID: 3953

NCBI Accession: NP_002294, NP_001003679, NP_001003680

UniProt: P48357

Pathways: JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour