Catalase antibody (AA 498-511)

Catalog No. ABIN364408

This Goat Polyclonal antibody specifically detects Catalase in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Dog, Monkey, Horse, Bat and Hamster.

Quick Overview for Catalase antibody (AA 498-511) (ABIN364408)

Target: Catalase (CAT)

Reactivity: Human, Mouse, Dog, Monkey, Horse, Bat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This Catalase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Catalase Antibody

Binding Specificity: AA 498-511

Brand: IHC-plus™

Specificity: Human CAT / Catalase.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Bat, Dog, Horse (100%) Elephant, Pig, Guinea pig (93%) Sheep, Goat, Bovine (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DKYNAEKPKNAIHT from an internal region of human CAT / Catalase (NP_001743.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Bat, Dog, Horse (100%), Elephant, Pig, Guinea pig (93%), Sheep, Goat, Bovine (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: CAT / Catalase

Background: Name/Gene ID: CAT

Synonyms: CAT, Catalase

Gene ID: 847

NCBI Accession: NP_001743

UniProt: P04040

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism