F12 antibody

Catalog No. ABIN364435

This Goat Polyclonal antibody specifically detects F12 in ELISA, IHC, IP, IHC (p) and IEP. It exhibits reactivity toward Human.

Quick Overview for F12 antibody (ABIN364435)

Target: F12 (Coagulation Factor XII (F12))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This F12 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoelectrophoresis (IEP)

Product Details anti-F12 Antibody

Brand: IHC-plus™

Specificity: Specific for Factor XII as demonstrated by immunodiffusion. A single precipitin arc was obtained with normal plasma and no reaction was observed with F. XII-deficient plasma.

Purification: Ammonium sulfate precipitation

Immunogen: Activated human Factor XII (F XIIa) purified from plasma.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IE, IHC, IHC-P (5 μg/mL), IP

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.4, 0.15 M sodium chloride, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for F12

Target: F12 (Coagulation Factor XII (F12))

Alternative Name: F12 / Factor XII

Background: Name/Gene ID: F12
Subfamily: Serine S1
Family: Protease

Synonyms: F12, Beta-factor XIIa part 2, Beta-factor XIIa part 1, Coagulation factor XII, F12 deficiency, Factor XII, Factor XII deficiency, HAE3, HAEX, Hageman factor, HAF

Gene ID: 2161

UniProt: P00748

Pathways: Complement System