CD72 antibody

Catalog No. ABIN371001

Product Details anti-CD72 Antibody

Target: CD72 (CD72 Molecule (CD72))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD72 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Specificity: This antibody recognises CD72.1/Lyb-2.1 (Mr. 45 kDa). It blocks binding of CD5 to CD72 on the B cell surface, which leads to inhibition of the positive signal resulting from CD5/CD72 pairing. (Ref.1) However, 10-1.D.2 can itself trigger a positive signal by binding CD72. (Ref.1-5)

Characteristics: Synonyms: Lyb-2, Ly-32, Ly32, B-Cell marker, B-cell differentiation antigen CD72, Lymphocyte antigen32

Purification: Purified

Immunogen: DBA/2 mouse spleen cells. (Ref. 1)

Clone: 10-1-D-2

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry. (Ref.1,2)Functional Assays: In vitro blocking and proliferation assays. (Ref.2,3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD72

Target: CD72 (CD72 Molecule (CD72))

Alternative Name: CD72 (CD72 Products)

Background: Murine CD72.1/Lyb-2.1, a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells, and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 (Ly-1) which is distributed on all T cells and a small number of B cells. (Ref.1,2)Synonyms: B-Cell marker, B-cell differentiation antigen CD72, Ly-32, Ly32, Lyb-2, Lymphocyte antigen 32

Gene ID: 100504743

UniProt: P21855

Pathways: BCR Signaling