Ly6C antibody

Catalog No. ABIN371039

The Rat Monoclonal anti-Ly6C antibody has been validated for FACS, IHC (fro) and Func. It is suitable to detect Ly6C in samples from Mouse.

Quick Overview for Ly6C antibody (ABIN371039)

Target: Ly6C (Ly-6C) (Ly-6C)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly6C antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: HK1-4

Product Details anti-Ly6C Antibody

Specificity: This antibody is specific to Murine Ly-6C (Mr. 18 kDa)

Characteristics: Synonyms: Lymphocyte antigen 6C2, Ly-6C2, Ly6C2, Mouse Macrophage Marker

Purification: Purified

Isotype: IgG2c

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.1,2)Immunohistochemistry on Frozen Sections. (Ref.5)In vitro Activation of T Lymphocytes. (Ref.1,2)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Ly6C

Target: Ly6C (Ly-6C) (Ly-6C)

Alternative Name: Ly6c

Background: Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes. (Ref.1-4)Synonyms: Ly-6C2, Ly6C2, Lymphocyte antigen 6C2, Mouse Macrophage Marker

Gene ID: 100041546

UniProt: P09568