Endoglin antibody

Catalog No. ABIN371132

Product Details anti-Endoglin Antibody

Target: Endoglin (ENG)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Endoglin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Characteristics: Synonyms: ENG, END, HHT1, ORW, ORW1

Purification: Purified

Immunogen: Inflamed mouse skin. (Ref. 1)

Clone: MJ7-18

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Identification and enumeration of CD105+ cells. (< / = 1 μg/10^6 cells). Immunohistochemistry on Frozen Sections. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105 / Endoglin (ENG Products)

Background: CD105/endoglin is a disulfide-linked type II transmembrane homodimer that is predominantly expressed on vascular endothelial cells. It is also found on pre-erythroblasts, macrophages and lymphoid and myeloid leukemic cells. (Ref.2) Both mouse and human CD105 exhibit > 70 % sequence homology in their cytoplasmic domains with type III Transforming Growth Factor beta (TGF-beta) receptor. (Ref.3) CD105 has been shown to be one of several receptors for the various isoforms of TGF-beta, which in turn is one of a family of proteins involved in regulation of cell differentiation, migration of cells and control of the immune response. (Ref.4)Synonyms: END, ENG, HHT1, ORW, ORW1

UniProt: Q63961