MHC, Class I antibody (FITC)

Catalog No. ABIN371395

Product Details anti-MHC, Class I Antibody (FITC)

Target: MHC, Class I

Reactivity: Chicken, Turkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC, Class I antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Characteristics: Synonyms: MHC class I-alpha, Major Histocompatibility Complex Class I

Clone: F21-2

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.1-4)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MHC, Class I

Target: MHC, Class I

Alternative Name: MHC Class I (MHC, Class I Products)

Synonyms: H-2K antibody, H-2K(d) antibody, H2-K antibody, K-f antibody, Y-FA antibody, YFV antibody, histocompatibility 2, K1, K region antibody, MHC class I antigen YF5 antibody, MHC class I antigen antibody, major histocompatibility complex, class I antibody, H2-K1 antibody, YF5 antibody, OVAR antibody, LOC512672 antibody

Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.1-3) Fibroblasts or neurons however only show a low level of antigen.Synonyms: HLA Class 1, MHC Class 1, Major Histocompatibility complex class I