CD43 antibody

Catalog No. ABIN371550

This anti-CD43 antibody is a Mouse Monoclonal antibody detecting CD43 in FACS, IHC (p) and IHC (fro). Suitable for Human.

Quick Overview for CD43 antibody (ABIN371550)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DF-T1

Product Details anti-CD43 Antibody

Specificity: This antibody recognizes CD43.

Characteristics: Synonyms: Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, SPN

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. Immunohistochemistry on Frozen and Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Preservative: Azide free

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43 / Leukosialin

Background: CD43 is one of the major glycoproteins of thymocytes and T lymphocytes, it plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response. CD43 is expressed by the majority of leucocytes, with the exception of most B cells. However CD43 expression is seen in some cases of B cell lymphocytic lymphoma and centrocytic lymphoma.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin

Gene ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity