GM-CSF antibody (Biotin)

Catalog No. ABIN371617

This Rat Monoclonal antibody specifically detects GM-CSF in FACS, IF, EIA and ELISA (Detection). It exhibits reactivity toward Human.

Quick Overview for GM-CSF antibody (Biotin) (ABIN371617)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA), ELISA (Detection)

Clone: BVD2-21C11

Product Details anti-GM-CSF Antibody (Biotin)

Specificity: This antibody is specific to Human Granulocyte/Macrophage-Colony Stimulating Factor (GM-CSF)1-4. The BVD2-21C11 antibody has been reported to cross react with GM-CSF from Rhesus monkey. (This is a Neutralizing antibody).

Characteristics: Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulatingfactor, Colony stimulating factor 2

Immunogen: E. coli-expressed human GM-CSF.

Isotype: IgG2a

Application Details

Application Notes: ELISA Detection: Biotinylated Monoclonal antibody ABIN371617 is the preferreddetection antibody in a Sandwich ELISA for quantifying human GM-CSF protein levels. (Ref.1-4). It should be paired with purified withpurified recombinant human GM-CSF as the standard. Immunofluorescence/Flow Cytometry: This antibody is useful for Intracytoplasmic stainingand Flow Cytometric analysis to identify and enumerate GM-CSF-positive cells within mixedcell populations. Recombinant human GM-CSF (Cat#ABIN371618 should be used as a Specificity Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

Gene ID: 1437

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin