DIABLO antibody

Catalog No. ABIN371649

The Mouse Monoclonal anti-DIABLO antibody (Clone 2D12) (ABIN371649) specifically detects DIABLO in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for DIABLO antibody (ABIN371649)

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DIABLO antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 2D12

Product Details anti-DIABLO Antibody

Specificity: This antibody is specific for Human SMAC/DIABLO.

Characteristics: Synonyms: Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, DirectIAP-binding protein with low pI

Purification: Purified

Isotype: IgG2b

Application Details

Application Notes: ELISA: 1/1,000-1/2,000. Western Blot: 1/5,000-1/10,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for DIABLO

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Alternative Name: DIABLO / SMAC

Background: SMAC/DIABLO (second mitochondria-derived activator of caspase/direct IAP-binding protein with low PI) is a mitochondrial protein that functions as a regulatory component during apoptosis. It is encoded by a nuclear gene and is subsequently imported into the mitochondria where the N-terminus is removed generating the mature form of the molecule. SMAC/DIABLO has been shown to neutralize the antiapoptotic effect of the inhibitor of apoptosis protein (IAP) family, a major group of apoptosis regulators that have been shown to directly block caspase activity thereby blocking apoptosis. (Ref.1-6)Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase

Gene ID: 56616

UniProt: Q9NR28

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity