Progesterone Receptor antibody (pSer294)

Catalog No. ABIN371835

This anti-Progesterone Receptor antibody is a Mouse Monoclonal antibody detecting Progesterone Receptor in WB and IHC (fro). Suitable for Human, Rat, Mouse and Non-Human Primate.

Quick Overview for Progesterone Receptor antibody (pSer294) (ABIN371835)

Target: Progesterone Receptor (PGR)

Reactivity: Human, Rat, Mouse, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Progesterone Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 608

Product Details anti-Progesterone Receptor Antibody

Binding Specificity: pSer294

Specificity: This antibody is specific for the ~90k PR-A isoform and the ~120k PR-B isoform phosphorylated at Ser294. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and non-Human Primates.
Species reactivity (tested):Human.

Characteristics: Synonyms: PR, PGR, NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3

Purification: Affinity Chromatography on Protein G.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser294of Human progesterone receptor.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1/1000. Immunohistochemitry (Frozen Sections, unpublished observations): 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Progesterone Receptor

Target: Progesterone Receptor (PGR)

Alternative Name: Progesterone Receptor

Background: There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000, Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) . Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.Synonyms: NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3, PGR, PR

Gene ID: 5241

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration