Acetylcholinesterase antibody (N-Term)

Catalog No. ABIN372290

The Rabbit Polyclonal anti-Acetylcholinesterase antibody has been validated for WB and IHC (p). It is suitable to detect Acetylcholinesterase in samples from Human.

Quick Overview for Acetylcholinesterase antibody (N-Term) (ABIN372290)

Target: Acetylcholinesterase (AChE)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetylcholinesterase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Acetylcholinesterase Antibody

Binding Specificity: N-Term

Specificity: This antibody recognizes Acetylcholinesterase (ACHE).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to N-terminal residues of Human Acetylcholinesterase (N- terminal extended acetylcholinesterase).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 20 μg/mL. Western Blot: 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.01 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: Acetylcholinesterase

Background: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.Synonyms: AChE, Acetylcholine Esterase, Acetylcholine-Esterase

Molecular Weight: 68 kDa.

Gene ID: 43

NCBI Accession: NP_000656

UniProt: P22303

Pathways: Skeletal Muscle Fiber Development