CA2 antibody (HRP)

Catalog No. ABIN372476

The Rabbit Polyclonal anti-CA2 antibody is suitable to detect CA2 in samples from Human and Rabbit. It has been validated for WB, IHC (p) and EIA.

Quick Overview for CA2 antibody (HRP) (ABIN372476)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Human, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-CA2 Antibody (HRP)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti Rabbit serum, as well as purified and partially purified Carbonic Anhydrase II (Human Erythrocytes).

Cross-Reactivity (Details): Species reactivity (expected):Rabbit.
Species reactivity (tested):Human.

Purification: Ion Exchange Chromatography.

Immunogen: Carbonic Anhydrase II (Human Erythrocytes).

Isotype: IgG

Application Details

Application Notes: ELISA: 1/1000-1/6000. Western blot. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2 containing 0.01 % Gentamicin Sulfate as preservative and 10 mg/mL BSA as stabilizer, before the addition of Glycerol to 40 %

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: Carbonic Anhydrase 2

Background: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20μM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.Synonyms: CA-II, CA2, CAC, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II

Gene ID: 760

NCBI Accession: NP_000058

UniProt: P00918