INA antibody

Catalog No. ABIN372587

The Chicken Polyclonal anti-INA antibody has been validated for WB, IF and IHC (fro). It is suitable to detect INA in samples from Human, Mouse and Rat.

Quick Overview for INA antibody (ABIN372587)

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reactivity: Human, Mouse, Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This INA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-INA Antibody

Specificity: Specific for the ~66k alpha Internexin protein. Minor bands at ~150k are probably covalent dimers and bands at ~50k represent Alpha-Internexin breakdown products.

Cross-Reactivity (Details): Species reactivity (expected):many other Mammals.
Species reactivity (tested):Human, Mouse and Rat.

Purification: Ig Fraction

Immunogen: Recombinant rat alpha-internexin expressed in and purified from E. coli.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot: 1/1000. Immunofluorescence: 1/500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for INA

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Alternative Name: alpha-Internexin

Background: Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).Synonyms: 66 kDa neurofilament protein, Alpha-Inx, INA, NEF5, NF66, Neurofilament-66

Gene ID: 9118

NCBI Accession: NP_116116

UniProt: Q16352