SLC6A1 antibody (C-Term)

Catalog No. ABIN372646

This Rabbit Polyclonal antibody specifically detects SLC6A1 in WB and IHC (fro). It exhibits reactivity toward Rat and Mouse.

Quick Overview for SLC6A1 antibody (C-Term) (ABIN372646)

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SLC6A1 Antibody

Binding Specificity: C-Term

Specificity: Specific for the ~67k GAT-1 protein. Immunolabeling is blocked by the peptide used as antigen.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide corresponding to amino acid residues from the C-terminal region of rat GAT-1.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000. Immunohistochemitsry: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for SLC6A1

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Alternative Name: GAT1 / SLC6A1

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).Synonyms: GABA transporter 1, GABATR, GABT1, GAT-1, Sodium- and chloride-dependent GABA transporter 1, Solute carrier family 6 member 1

Gene ID: 79212

NCBI Accession: NP_077347

UniProt: P23978