GLRA1 antibody (N-Term)

Catalog No. ABIN372657

This anti-GLRA1 antibody is a Rabbit Polyclonal antibody detecting GLRA1 in WB, DB and IHC (fro). Suitable for Human, Rat and Mouse.

Quick Overview for GLRA1 antibody (N-Term) (ABIN372657)

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLRA1 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GLRA1 Antibody

Binding Specificity: N-Term

Specificity: This antibody recognizes the ~48k alpha-1 and alpha-2 Subunits of the Glycine Receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other Glycine Receptor subunits.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide from the N-terminus region of the alpha-1 subunit of rat Glycine Receptor.

Isotype: IgG

Application Details

Application Notes: Dot blot: 1/1000. Western blot: 1/1000. Immunohistochemistry: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 100 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Buffer: 5 mM Ammonium Bicarbonate.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: After reconstitution: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for GLRA1

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Alternative Name: Glycine Receptor alpha-1

Background: Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001, Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).Synonyms: GLRA1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit

Gene ID: 2741

NCBI Accession: NP_000162

UniProt: P23415

Pathways: Synaptic Membrane