MARCKS antibody (pSer152, pSer156)

Catalog No. ABIN372663

The Rabbit Polyclonal anti-MARCKS antibody (ABIN372663) specifically detects MARCKS in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Chicken and Xenopus laevis samples.

Quick Overview for MARCKS antibody (pSer152, pSer156) (ABIN372663)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MARCKS Antibody

Binding Specificity: pSer152, pSer156

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Chicken, Human, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser152,156 of MARCKS.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS

Background: Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL

Gene ID: 10116

UniProt: P30009