Metabotropic Glutamate Receptor 1 antibody (C-Term)

Catalog No. ABIN372668

The Rabbit Polyclonal anti-Metabotropic Glutamate Receptor 1 antibody is suitable to detect Metabotropic Glutamate Receptor 1 in samples from Rat. It has been validated for WB and IHC (fro).

Quick Overview for Metabotropic Glutamate Receptor 1 antibody (C-Term) (ABIN372668)

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Metabotropic Glutamate Receptor 1 Antibody

Binding Specificity: C-Term

Specificity: This antibody is specific for the ~125k monomer and the ~250k mGluR1a dimer. Immunolabeling blocked by preadsorption of antibody with the peptide used to generate the antibody.

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide from the C-terminal region of Rat mGluR1a.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for Metabotropic Glutamate Receptor 1

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Alternative Name: mGluR1a / GRM1a

Background: The metabotropic Glutamate Receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004, Francesconi et al., 2004, Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003).Synonyms: Gprc1a, Metabotropic glutamate receptor 1, Mglur1

Gene ID: 24414

NCBI Accession: NP_001107802

UniProt: P23385