STXBP1 antibody (pSer515)

Catalog No. ABIN372671

Product Details anti-STXBP1 Antibody

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Binding Specificity: pSer515

Reactivity: Human, Mouse, Rat, Cow, Dog, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STXBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognizes ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser515 of Munc-18.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry: 1/400. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20°C.

Expiry Date: 12 months

Target Details for STXBP1

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Alternative Name: STXBP1 / UNC18A (STXBP1 Products)

Synonyms: MUNC18-1 antibody, NSEC1 antibody, P67 antibody, RBSEC1 antibody, UNC18 antibody, AI317162 antibody, AI326233 antibody, MMS10-G antibody, Ms10g antibody, Munc-18a antibody, Munc18-1 antibody, N-sec1 antibody, Rb-sec1 antibody, Sxtbp1 antibody, Unc18-1 antibody, Unc18h antibody, nsec1 antibody, fj43h10 antibody, stxbp1 antibody, wu:fj36d12 antibody, wu:fj43h10 antibody, zgc:114171 antibody, rop antibody, unc18 antibody, hunc18 antibody, rbsec1 antibody, munc18-1 antibody, MGC146482 antibody, ANC18HA antibody, NSEC1A antibody, Sec1 antibody, n-sec1 antibody, nSec1 antibody, rbSec1 antibody, rbSec1A antibody, rbSec1B antibody, UNC18A antibody, si:rp71-10d23.3 antibody, syntaxin binding protein 1 antibody, syntaxin binding protein 1a antibody, syntaxin binding protein 1 L homeolog antibody, syntaxin binding protein 1b antibody, STXBP1 antibody, Stxbp1 antibody, stxbp1a antibody, stxbp1 antibody, Tb09.160.0780 antibody, stxbp1.L antibody, stxbp1b antibody

Background: Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).
Alternate names: N-Sec1, Syntaxin-binding protein 1, Unc-18 homolog, Unc-18-1, Unc-18A

Gene ID: 25558

NCBI Accession: NP_037170

UniProt: P61765

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport