STXBP1 antibody (pSer515)

Catalog No. ABIN372671

The Rabbit Polyclonal anti-STXBP1 antibody is suitable to detect STXBP1 in samples from Human, Mouse, Rat, Cow, Dog, Chicken and Xenopus laevis. It has been validated for WB and IHC (fro).

Quick Overview for STXBP1 antibody (pSer515) (ABIN372671)

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STXBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-STXBP1 Antibody

Binding Specificity: pSer515

Specificity: This antibody recognizes ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser515 of Munc-18.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry: 1/400. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20°C.

Expiry Date: 12 months

Target Details for STXBP1

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Alternative Name: STXBP1 / UNC18A

Background: Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).
Alternate names: N-Sec1, Syntaxin-binding protein 1, Unc-18 homolog, Unc-18-1, Unc-18A

Gene ID: 25558

NCBI Accession: NP_037170

UniProt: P61765

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport