NEFL antibody

Catalog No. ABIN372673

This Chicken Polyclonal antibody specifically detects NEFL in WB, IF and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse, Cow and Chicken.

Quick Overview for NEFL antibody (ABIN372673)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human, Rat, Mouse, Cow, Chicken

Host: Chicken

Clonality: Polyclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NEFL Antibody

Specificity: This antibody recognizes the ~68k Neurofilament L protein.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Chicken, Mouse and Rat.

Purification: Ig Fraction

Immunogen: Preparation of bovine spinal cord intermediate filaments.

Isotype: Ig Fraction

Application Details

Application Notes: Western blot: 1/1000. Immunofluorescence: 1/5000. Immunohistochemistry: This antibody works well on Frozen Sections, Cells in tissueculture and on mildly Formalin Fixed Histological Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: Neurofilament L (68kDa)

Background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (Ref.1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (Ref.2).Synonyms: 68 kDa neurofilament protein, NEFL, NF-L, NF68, Neurofilament L

Gene ID: 83613

NCBI Accession: NP_113971

UniProt: P19527