GRIN1/NMDAR1 antibody (Splice Variant C2')

Catalog No. ABIN372677

The Rabbit Polyclonal anti-GRIN1/NMDAR1 antibody has been validated for WB and IHC (fro). It is suitable to detect GRIN1/NMDAR1 in samples from Human, Rat, Mouse and Cow. There are 2+ publications available.

Quick Overview for GRIN1/NMDAR1 antibody (Splice Variant C2') (ABIN372677)

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivity: Human, Rat, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN1/NMDAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GRIN1/NMDAR1 Antibody

Binding Specificity: Splice Variant C2'

Specificity: This antibody recognizes the ~120k NR1 subunit of the NMDA receptor containing the C2' splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2' insert.

Cross-Reactivity (Details): Species reactivity (expected):Bovine and Human.
Species reactivity (tested):Mouse and Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide from the NR1 subunit, C2' splice variant insert of rat NMDA Receptor.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000-1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Buffer: 5 mM Ammonium Bicarbonate.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-GRIN1/NMDAR1 antibody (ABIN372677)

Brady, Diaz, Iuso, Everett, Valenzuela, Caldwell: "Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 3, pp. 1062-7, (2013) (PubMed).

Song, Kaczmarek: "Modulation of Kv3.1b potassium channel phosphorylation in auditory neurons by conventional and novel protein kinase C isozymes." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15582-91, (2006) (PubMed).

Target Details for GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Alternative Name: NMDA Receptor 1

Background: The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). Increased membrane surface expression of the NMDAR, NR1-Subunit has been associated with synaptic plasticity (Grosshans et al., 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994, Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.Synonyms: GRIN1, Glutamate [NMDA] receptor subunit zeta-1, NMDAR1

Gene ID: 24408

NCBI Accession: NP_058706

UniProt: P35439

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity