STK10-A antibody (pSer482, pSer486, pSer490)

Catalog No. ABIN372704

The Rabbit Polyclonal anti-STK10-A antibody has been validated for WB. It is suitable to detect STK10-A in samples from Human, Mouse, Zebrafish (Danio rerio), Rat and Xenopus laevis.

Quick Overview for STK10-A antibody (pSer482, pSer486, pSer490) (ABIN372704)

Target: STK10-A (Serine/threonine Kinase 10 (STK10-A))

Reactivity: Human, Mouse, Zebrafish (Danio rerio), Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STK10-A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-STK10-A Antibody

Binding Specificity: pSer482, pSer486, pSer490

Specificity: This antibody recognizes the ~120k PLKK protein phosphorylated at Ser482,486,490.

Cross-Reactivity (Details): Species reactivity (expected):Human, Mouse, Rat and Zebrafish.
Species reactivity (tested):Xenopus.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser482,486,490 of Xenopus polo-like kinase kinase (PLKK).

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for STK10-A

Target: STK10-A (Serine/threonine Kinase 10 (STK10-A))

Alternative Name: PLKK1

Background: Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.Synonyms: Polo-like kinase kinase 1, stk10, xPlkk1

Gene ID: 373731

NCBI Accession: NP_001079164

UniProt: Q9YHC9