SNAP25 antibody (C-Term)

Catalog No. ABIN372760

This Goat Polyclonal antibody specifically detects SNAP25 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Rat, Mouse and Dog.

Quick Overview for SNAP25 antibody (C-Term) (ABIN372760)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Human, Rat, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SNAP25 Antibody

Binding Specificity: C-Term

Specificity: Recognizes Synaptosomal Associated Protein 25 (SNAP25).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide (DEANQRATKMLGSG) -KLH conjugated from C-terminus of Human SNAP25.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/32000. Western Blot: 0.01-0.03 μg/mL. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris Saline Buffer, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP25

Background: SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre-synaptic nerve terminal protein that plays an essential role in synaptic vesicle fusion and exocytosis. It is part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. SNAP25 is involved in the molecular regulation of neurotransmitter release. It is thought to play an important role in the synaptic function of specific neuronal systems and it associates with proteins involved in vesicle docking and membrane fusion. SNAP25 exists as two alternatively spliced isoforms, SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and they differ by 9 amino acids in the central portion of the protein.Synonyms: RIC4, SNAP, SNAP-25, SUP, Super Protein, Synaptosomal-associated 25 kDa protein, Synaptosomal-associated protein 25, ric-4

Gene ID: 6616

NCBI Accession: NP_003072

UniProt: P60880

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport