GAPDH antibody (AA 73-87)

Catalog No. ABIN372846

The Rabbit Polyclonal anti-GAPDH antibody (ABIN372846) specifically detects GAPDH in WB and IHC (p). The antibody is reactive with Human, Mouse, Rat, Pig, Cat, Drosophila melanogaster, Macaque and Chimpanzee samples.

Quick Overview for GAPDH antibody (AA 73-87) (ABIN372846)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Pig, Cat, Drosophila melanogaster, Macaque, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GAPDH Antibody

Binding Specificity: AA 73-87

Specificity: This antibody reacts to Glyceraldehyde 3-phosphate Dehydrogenase (GAPDH).

Purification: Affinity chromatography on Protein G

Immunogen: Amino acids 73-87 (PITIFQERDPSKIKW) of glyceraldehyde 3-phosphate dehydrogenase protein

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 5 μg/mL. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS conatining 0.05 % BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-28 °C for longer.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase

Gene ID: 2597

NCBI Accession: NP_002037

UniProt: P04406