PLIN3 antibody (N-Term)

Catalog No. ABIN372848

The Rabbit Polyclonal anti-PLIN3 antibody is suitable to detect PLIN3 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for PLIN3 antibody (N-Term) (ABIN372848)

Target: PLIN3 (Perilipin 3 (PLIN3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLIN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLIN3 Antibody

Binding Specificity: N-Term

Specificity: This antibody recognizes Mannose-6-phosphate Receptor Binding Protein 1 (M6PRBP1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide - KLH conjugated corresponding to 14 amino acids from near the amino terminus of Human TIP47

Isotype: IgG

Application Details

Application Notes: Western blot. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This M6PRBP1 antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PLIN3

Target: PLIN3 (Perilipin 3 (PLIN3))

Alternative Name: TIP47 / M6PRBP1

Background: Mannose 6-phosphate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. M6PRBP1 interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo.Synonyms: 47 kDa mannose 6-phosphate receptor-binding protein, Cargo selection protein TIP47, Mannose-6-phosphate receptor-binding protein 1, Placental protein 17

Gene ID: 10226

NCBI Accession: NP_001157661

UniProt: O60664