B-Cell Linker antibody (C-Term)

Catalog No. ABIN374092

This Goat Polyclonal antibody specifically detects B-Cell Linker in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog and Pig.

Quick Overview for B-Cell Linker antibody (C-Term) (ABIN374092)

Target: B-Cell Linker (BLNK)

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This B-Cell Linker antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-B-Cell Linker Antibody

Binding Specificity: C-Term

Sequence: C-KDSTRLKYAV KVS

Specificity: Detects B-cell linker protein/BLNK at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_037446.1, NP_001107566.1 Genename: BLNK

Application Details

Application Notes: Peptide ELISA: BLNK antibody Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 70 kDa band observed in lysates of cell line Daudi(calculated MW of 50.0 kDa according to 037446.1). The observed molecular weightcorresponds to how SLP-65 is expected to run. However, when transiently expressed inHEK293, a 50 kDa band is observed.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: B-Cell Linker Protein / BLNK

Background: BLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck.Synonyms: B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, BASH, Cytoplasmic adapter protein, SLP-65, SLP65, Src homology 2 domain-containing leukocyte protein of 65 kDa

Gene ID: 29760

NCBI Accession: NP_037446

UniProt: Q8WV28

Pathways: BCR Signaling