BAG2 antibody (C-Term)

Catalog No. ABIN374110

This Goat Polyclonal antibody specifically detects BAG2 in WB. It exhibits reactivity toward Human.

Quick Overview for BAG2 antibody (C-Term) (ABIN374110)

Target: BAG2 (BCL2-Associated Athanogene 2 (BAG2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BAG2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BAG2 Antibody

Binding Specificity: C-Term

Sequence: CSKTLQQNAE SRFN

Specificity: This antibody detects BAG2 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_004273.1. Genename: BAG2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 8000. Western blot: 0.1-0.3 μg/mL. Approx 25 kDa band observed in Human HeLa lysates(calculated MW of 23.5 kDa according to NP_004273.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for BAG2

Target: BAG2 (BCL2-Associated Athanogene 2 (BAG2))

Alternative Name: BAG2

Background: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.Synonyms: BAG family molecular chaperone regulator 2, BAG-2, Bcl-2-associated athanogene 2

Gene ID: 9532

NCBI Accession: NP_004273

UniProt: O95816