DDAH1 antibody (C-Term)

Catalog No. ABIN374149

This Goat Polyclonal antibody specifically detects DDAH1 in WB. It exhibits reactivity toward Human and Cow.

Quick Overview for DDAH1 antibody (C-Term) (ABIN374149)

Target: DDAH1 (Dimethylarginine Dimethylaminohydrolase 1 (DDAH1))

Reactivity: Human, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This DDAH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDAH1 Antibody

Binding Specificity: C-Term

Sequence: TCCSVLINKK VDS

Specificity: This antibody is expected to recognize both reported isoforms of DDAH1 (NP_036269.1and NP_001127917.1).

Cross-Reactivity (Details): Species reactivity (expected):Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_036269.1, NP_001127917.1 Genename: DDAH1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.1-0.3 μg/mL. Approx 37 kDa band observed in human kidney, liver andpancreas lysates (calculated MW of 31.7 kDa according to NP_036269.1). The observedmolecular weight corresponds to earlier findings in literature with different antibodies(Pullamsetti et al, FASEB J.2005 Jul,19(9): 1175-7, PMID: 15827267).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DDAH1

Target: DDAH1 (Dimethylarginine Dimethylaminohydrolase 1 (DDAH1))

Alternative Name: DDAH1

Background: DDAH1 belongs to the dimethylarginine dimethylaminohydrolase (DDAH) family. This enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.Synonyms: DDAH, DDAH-1, DDAHI, Dimethylargininase-1, Dimethylarginine dimethylaminohydrolase 1, N(G), N(G)-dimethylarginine dimethylaminohydrolase 1

Gene ID: 23576

NCBI Accession: NP_036269

UniProt: O94760