Annexin A11 antibody (N-Term)

Catalog No. ABIN374162

The Goat Polyclonal anti-Annexin A11 antibody is suitable to detect Annexin A11 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Annexin A11 antibody (N-Term) (ABIN374162)

Target: Annexin A11 (ANXA11)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Annexin A11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Annexin A11 Antibody

Binding Specificity: N-Term

Sequence: YPGYPPPPGG YPP-C

Specificity: This antibody detects Annexin A11 / ANXA11 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from N-Terminus of the protein sequence according to NP_001148.1, NP_665875.1, NP_665876.1. Genename: ANXA11

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 55 kDa band seen in HeLa lysate (Predicted MW ofapprox.55 kDa according to NP_001148.1). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Placenta shows strongstaining of Basement Membrane in trophoblasts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Annexin A11

Target: Annexin A11 (ANXA11)

Alternative Name: Annexin A11 / ANXA11

Background: Annexin A11 is a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 54 kD antigen recognized by sera from patients with various autoimmune diseases. Transcript variants encoding the same isoform have been identified.Synonyms: 56 kDa autoantigen, ANX11, Annexin XI, Annexin-11, CAP-50, Calcyclin-associated annexin 50

Gene ID: 311

NCBI Accession: NP_001148

UniProt: P50995