UBE2C antibody (C-Term)

Catalog No. ABIN374251

This Goat Polyclonal antibody specifically detects UBE2C in WB, IF, IP and EIA. It exhibits reactivity toward Human.

Quick Overview for UBE2C antibody (C-Term) (ABIN374251)

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-UBE2C Antibody

Binding Specificity: C-Term

Sequence: C-QETYSKQVTS QEP

Specificity: This antibody is expected to recognise all reported isoforms.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1. Genename: UBE2C

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approximately 20KDa band was detected in mitotic (i. eNocodazole arrested) HeLa whole cell lysates. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates usingprotein-G dynabeads. Immunofluorescence: Stained mitotic and late G2 Hela cells, withvery faint or no signal in G1 and S phase cells (fixed using paraformaldehyde, primaryconcentration 2.5 μg/mL, using FITC anti-Goat).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for UBE2C

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Alternative Name: UBE2C / UBCH10

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression.Synonyms: Ubiquitin carrier protein C, Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C

Gene ID: 11065

NCBI Accession: NP_008950

UniProt: O00762

Pathways: Ubiquitin Proteasome Pathway