IRF5 antibody (C-Term)

Catalog No. ABIN374255

The Goat Polyclonal anti-IRF5 antibody is suitable to detect IRF5 in samples from Human, Mouse, Rat and Cow. It has been validated for WB, IP, IHC (p), ChIP and EIA.

Quick Overview for IRF5 antibody (C-Term) (ABIN374255)

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This IRF5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA)

Product Details anti-IRF5 Antibody

Binding Specificity: C-Term

Sequence: C-QGPWPMHPAG MQ

Specificity: This antibody is expected to recognize all four reported isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1. Genename: IRF5

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 57 kDa band observed in lysates of cell line A549. Intransfected HEK293 transiently expressing IRF5 a strong band of approx.67 kDa isobserved. This band is not observed in the non-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for IRF5

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Alternative Name: IRF5

Background: Interferon regulatory factors (IRFs) are transcription factors that possess a novel helix turn helix DNA binding motif and mediate virus and interferon (IFN) induced signaling pathways. The IRF5 protein is structurally related to IRF6. Like other IRFs, IRF5 and IRF6 contain 5 conserved tryptophan residues in the 115 amino acid N terminal DNA binding domain. IRF5 has a central IRF association domain (IAD) and, like IRF7 and IRF8, is induced by IFNA/IFNB stimulation. IRF5 has a type I IFN transactivation potential similar to that of IRF1. IRF3 and IRF5 form heterodimers and are both required for effective induction of IFNA gene transcription.Synonyms: IRF-5, Interferon regulatory factor 5

Gene ID: 3663

NCBI Accession: NP_002191

UniProt: Q13568

Pathways: TLR Signaling, Autophagy