NDC80 antibody (C-Term)

Catalog No. ABIN374264

The Goat Polyclonal anti-NDC80 antibody is suitable to detect NDC80 in samples from Human. It has been validated for WB.

Quick Overview for NDC80 antibody (C-Term) (ABIN374264)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NDC80 Antibody

Binding Specificity: C-Term

Sequence: C-YEKKATLIKS SEE

Specificity: This antibody detects NDC80 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_006092.1 Genename: NDC80

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 75-80 kDa band observed in Hela lysate (predicted MWof 70 kDa according to NP_006092).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: NDC80

Background: HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.Synonyms: HEC, HEC1, KNTC2

Gene ID: 10403

NCBI Accession: NP_006092

UniProt: O14777

Pathways: Maintenance of Protein Location