EZH1 antibody (N-Term)

Catalog No. ABIN374284

This Goat Polyclonal antibody specifically detects EZH1 in WB. It exhibits reactivity toward Human, Mouse and Cow.

Quick Overview for EZH1 antibody (N-Term) (ABIN374284)

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Reactivity: Human, Mouse, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This EZH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EZH1 Antibody

Binding Specificity: N-Term

Sequence: EIPNPPTSKC ITY

Specificity: This antibody detects EZH1 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_001982.2 Genename: EZH1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 75-80 kDa band seen in MOLT-4 lysate [Predicted MW of80 kDa according to NP_001982].
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % sodium azide, 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EZH1

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Alternative Name: EZH1

Background: EZH1 is a human homolog of the Drosophila gene Enhancer of zeste E(z), a member of the polycomb group of transcriptional repressors. In mice, EZH1 is a regulator of homeotic gene expression implicated in the assembly of repressive protein complexes in chromatin. The strong sequence conservation between species suggests potential role for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that stably maintain heterochromatin.Synonyms: ENX-2, Enhancer of zeste homolog 1, Histone-lysine N-methyltransferase EZH1, KIAA0388

Gene ID: 2145

NCBI Accession: NP_001982

UniProt: Q92800